Electron transport in mitochondria isolated from the flagellate Polytomella caeca

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Electron transport in mitochondria isolated from the flagellate Polytomella caeca.

1. Mitochondria isolated from Polytomella caeca contain cytochromes b, c+c(1) and a+a(3) and several flavoprotein species. 2. Electron transport is inhibited by antimycin A, rotenone, piericidin A and cyanide. 3. Spectral data indicate that antimycin A inhibits the reoxidation of reduced cytochrome b. 4. Various types of flavoprotein are characterized by simultaneous spectrophotometric and fluo...

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The properties and subcellular localization of acid phosphatases in the colourless alga, Polytomella caeca.

The acid £-nitrophenyl phosphatase of homogenates of Polytomella caeca is a latent acid hydrolase, which is partially inhibited by NaF. Its distribution profile in density gradients (which is similar to that of naphthyl AS-TR phosphatase) suggests that this enzyme is partially lysosomal in location. Cytochemical evidence for the localization of acid phosphatases in fine subcellular structures i...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1968

ISSN: 0306-3283

DOI: 10.1042/bj1070829